Integration of Untargeted and Pseudotargeted Metabolomics for Authentication of Three Shrimp Species Using UHPLC-Q-Orbitrap

In this work, an untargeted and pseudotargeted metabolomics combination approach was used for authentication of three shrimp species (Litopenaeus vanmamei, Penaeus japonicus, monodon). The monophasic extraction-based enabled comprehensive-coverage high-throughput analysis tissue revealed 26 potential markers. confirmed 21 markers (including 9 key markers), which realized at least putative identification. markers, as well were to develop PLS-DA models, correctly classifying 60/60 testing samples. Furthermore, DD-SIMCA models integrated based on the with 59/60 20/20 samples that involved uninvolved in model training classified. results demonstrated authentication.